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apc conjugated anti cd82  (Miltenyi Biotec)


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    Miltenyi Biotec apc conjugated anti cd82
    Apc Conjugated Anti Cd82, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc+conjugated+anti+cd82/CD82+Antibody%2C+anti-human%2C+REAfinity/pmc04983081__mmc2-348-49-52
    Average 93 stars, based on 10 article reviews
    apc conjugated anti cd82 - by Bioz Stars, 2026-10
    93/100 stars

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    other:

    Article Title: Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle
    Article Snippet: Cells were then stained with streptavidin-PE/Cy5 (1:200, BD Pharmingen, #554062) or anti-Goat IgG-PE (1:200, R&D, #705-116-147) for 30 min at 4°C in the dark.

    Staining:

    Article Title: Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle
    Article Snippet: Cells were then stained with streptavidin-PE/Cy5 (1:200, BD Pharmingen, #554062) or anti-Goat IgG-PE (1:200, R&D, #705-116-147) for 30 min at 4°C in the dark. .. Primary antibodies used for cell staining were VioBright FITC- or PE-conjugated anti-CD56 (1:20, Miltenyi Biotec, #130-104-944 or 130-090-755), FITC- or PE/Cy7-conjugated anti-CD56 (1:40, BD Pharmingen, #335791), biotinylated anti-PDGFRa (2.5 μg/ml, R&D, #BAF322), BB515-conjugated anti-PDGFRa (1:40, BD Pharmingen, #564594), PE- or Alexa Fluor 647-conjugated anti-CD82 (1:40, BioLegend, #342104 or 342108), APC-conjugated anti-CD82 (1:200, Miltenyi Biotec, #130-101-311), PE-conjugated anti-CD274 (1:40, BioLegend, #329706), PE-conjugated anti-CD318 (1:40, BioLegend, #324006), PE-conjugated anti-CD201 (1:40, BioLegend, #351904), PE-conjugated anti-CD141 (1:40, BioLegend, #344104), and anti-PAR1 (5 μg/ml, R&D, #AF3855). .. Stained cells were analyzed by FACSVerse, FACSVantage SE (BD Biosciences), or MoFlo Astrios (Beckman Coulter).



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    Scheme outlining the key steps of the study. hiPSCs were injected into TA muscles of NSG mice, and teratomas were harvested two months later. Tissue was digested, and skeletal myogenic progenitor cells co-expressing <t>CD82,</t> ERBB3, and NGFR were sorted by FACS, cultured in vitro, tested for differentiation into myotubes, and, in parallel, transplanted into tibialis anterior (TA) muscles of injured NSG-mdx 4Cv mice following X-ray and cardiotoxin injection. TA muscle tissue was harvested at various time points, and stained sections were analyzed with different antibodies to assess human cell contribution, muscle regeneration, and fiber type differentiation.
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    Scheme outlining the key steps of the study. hiPSCs were injected into TA muscles of NSG mice, and teratomas were harvested two months later. Tissue was digested, and skeletal myogenic progenitor cells co-expressing <t>CD82,</t> ERBB3, and NGFR were sorted by FACS, cultured in vitro, tested for differentiation into myotubes, and, in parallel, transplanted into tibialis anterior (TA) muscles of injured NSG-mdx 4Cv mice following X-ray and cardiotoxin injection. TA muscle tissue was harvested at various time points, and stained sections were analyzed with different antibodies to assess human cell contribution, muscle regeneration, and fiber type differentiation.
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    Scheme outlining the key steps of the study. hiPSCs were injected into TA muscles of NSG mice, and teratomas were harvested two months later. Tissue was digested, and skeletal myogenic progenitor cells co-expressing <t>CD82,</t> ERBB3, and NGFR were sorted by FACS, cultured in vitro, tested for differentiation into myotubes, and, in parallel, transplanted into tibialis anterior (TA) muscles of injured NSG-mdx 4Cv mice following X-ray and cardiotoxin injection. TA muscle tissue was harvested at various time points, and stained sections were analyzed with different antibodies to assess human cell contribution, muscle regeneration, and fiber type differentiation.
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    apc conjugated anti cd82  (Miltenyi Biotec)
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    Miltenyi Biotec apc conjugated anti cd82
    Scheme outlining the key steps of the study. hiPSCs were injected into TA muscles of NSG mice, and teratomas were harvested two months later. Tissue was digested, and skeletal myogenic progenitor cells co-expressing <t>CD82,</t> ERBB3, and NGFR were sorted by FACS, cultured in vitro, tested for differentiation into myotubes, and, in parallel, transplanted into tibialis anterior (TA) muscles of injured NSG-mdx 4Cv mice following X-ray and cardiotoxin injection. TA muscle tissue was harvested at various time points, and stained sections were analyzed with different antibodies to assess human cell contribution, muscle regeneration, and fiber type differentiation.
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    Average 93 stars, based on 1 article reviews
    apc conjugated anti cd82 - by Bioz Stars, 2026-10
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    		  Buy from Supplier

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    Scheme outlining the key steps of the study. hiPSCs were injected into TA muscles of NSG mice, and teratomas were harvested two months later. Tissue was digested, and skeletal myogenic progenitor cells co-expressing CD82, ERBB3, and NGFR were sorted by FACS, cultured in vitro, tested for differentiation into myotubes, and, in parallel, transplanted into tibialis anterior (TA) muscles of injured NSG-mdx 4Cv mice following X-ray and cardiotoxin injection. TA muscle tissue was harvested at various time points, and stained sections were analyzed with different antibodies to assess human cell contribution, muscle regeneration, and fiber type differentiation.

    Journal: Cells

    Article Title: Long-Term Engraftment and Satellite Cell Expansion from Human PSC Teratoma-Derived Myogenic Progenitors

    doi: 10.3390/cells14151150

    Figure Lengend Snippet: Scheme outlining the key steps of the study. hiPSCs were injected into TA muscles of NSG mice, and teratomas were harvested two months later. Tissue was digested, and skeletal myogenic progenitor cells co-expressing CD82, ERBB3, and NGFR were sorted by FACS, cultured in vitro, tested for differentiation into myotubes, and, in parallel, transplanted into tibialis anterior (TA) muscles of injured NSG-mdx 4Cv mice following X-ray and cardiotoxin injection. TA muscle tissue was harvested at various time points, and stained sections were analyzed with different antibodies to assess human cell contribution, muscle regeneration, and fiber type differentiation.

    Article Snippet: The dissociated cells were incubated with antibodies APC anti-CD82 (FAB4616A, R&D Systems, Minneapolis, MN, USA; RRID: AB_2076404), PE anti-ERBB3 (304706, BioLegend, San Diego, CA, USA; RRID: AB_2099569), and PECy7 anti-NGFR (Cat# 562122, BD Biosciences, San Jose, CA, USA; RRID: AB_10894762); each at 0.5 uL per 1 million cells) in FACS buffer for one hour on ice.

    Techniques: Injection, Muscles, Expressing, Cell Culture, In Vitro, Staining

    ( A ) Morphology of teratomas formed in TA muscles of NSG mice two months after injection of hiPSCs. ( B ) Isolation of skeletal myogenic progenitor cells co-expressing CD82, ERBB3, and NGFR by FACS from TA teratomas. ( C ) Expression levels of various myogenic and fibroblastic markers under different growth factor conditions, as determined by RNA sequencing, presenting mean ratio normalized counts. ( D ) Morphology of isolated skeletal myogenic cells cultured at different passages. PAX7 staining of cells in the proliferative phase at confluency, from the same passage used for transplantation. ( E ) MyoD, Myogenin, MF-20, and PAX7 staining of teratoma-derived skeletal myogenic cells in differentiation medium at passage 3, highlighting their capacity to form myotubes. ( F ) Percentage of cells positive for PAX7 in proliferative and differentiation media, and for MyoD, Myogenin, and MF-20 in differentiation medium. Data were obtained from five fields per sample across three replicates.

    Journal: Cells

    Article Title: Long-Term Engraftment and Satellite Cell Expansion from Human PSC Teratoma-Derived Myogenic Progenitors

    doi: 10.3390/cells14151150

    Figure Lengend Snippet: ( A ) Morphology of teratomas formed in TA muscles of NSG mice two months after injection of hiPSCs. ( B ) Isolation of skeletal myogenic progenitor cells co-expressing CD82, ERBB3, and NGFR by FACS from TA teratomas. ( C ) Expression levels of various myogenic and fibroblastic markers under different growth factor conditions, as determined by RNA sequencing, presenting mean ratio normalized counts. ( D ) Morphology of isolated skeletal myogenic cells cultured at different passages. PAX7 staining of cells in the proliferative phase at confluency, from the same passage used for transplantation. ( E ) MyoD, Myogenin, MF-20, and PAX7 staining of teratoma-derived skeletal myogenic cells in differentiation medium at passage 3, highlighting their capacity to form myotubes. ( F ) Percentage of cells positive for PAX7 in proliferative and differentiation media, and for MyoD, Myogenin, and MF-20 in differentiation medium. Data were obtained from five fields per sample across three replicates.

    Article Snippet: The dissociated cells were incubated with antibodies APC anti-CD82 (FAB4616A, R&D Systems, Minneapolis, MN, USA; RRID: AB_2076404), PE anti-ERBB3 (304706, BioLegend, San Diego, CA, USA; RRID: AB_2099569), and PECy7 anti-NGFR (Cat# 562122, BD Biosciences, San Jose, CA, USA; RRID: AB_10894762); each at 0.5 uL per 1 million cells) in FACS buffer for one hour on ice.

    Techniques: Muscles, Injection, Isolation, Expressing, RNA Sequencing, Cell Culture, Staining, Transplantation Assay, Derivative Assay